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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be observed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial purpose in endorsing cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two additional splice variants ended up noted but detailed nucleotide sequence facts will not be but available.

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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded from the antibody.

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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...

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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the absolute best effects are...

Antibodies sure to the blocking peptide no more bind on the epitope over the concentrate on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.

Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins show unique expression and degradation designs which contribute to your temporal coordination of every mitotic occasion. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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